Trypanothione synthetase
WebBackground: Resistance to antimonials is a fundamental determinant of treatment failure in anthroponotic cutaneous leishmaniasis (ACL). Detection of reliable molecular markers to … WebDec 25, 2009 · Trypanothione synthetase (TryS; EC 6.3.1.9; spermidine/glutathionylspermidine:glutathione ligase (ADP-forming)) is one such target. …
Trypanothione synthetase
Did you know?
WebTrypanothione biosynthesis requires ATP-dependent conjugation of glutathione (GSH) to the two terminal amino groups of spermidine by glutathionylspermidine synthetase (GspS) … WebDOI: 10.1016/S0166-6851(03)00176-2 Corpus ID: 11475314; Properties of trypanothione synthetase from Trypanosoma brucei. @article{Oza2003PropertiesOT, title={Properties of trypanothione synthetase from Trypanosoma brucei.}, author={Sandra L. Oza and Mark R. Ariyanayagam and Niall Aitcheson and Alan H. Fairlamb}, journal={Molecular and …
WebDL-Buthionine-(S,R)-sulfoximine has also been used: to induce oxidative stress in Starlings; to deplete choroidal glutathione (GSH) in two-day-old rat pups; to test its inhibitory effect on the enzymes, γ-glutamylcysteine synthetase and trypanothione synthetase (TryS) from T. … WebRegulation of intracellular pH (pHi) was investigated in Trypanosoma cruzi amastigotes and trypomastigotes using 2′,7′-bis-(carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF). pHi …
In enzymology, a trypanothione synthase (EC 6.3.1.9) is an enzyme that catalyzes the chemical reaction glutathione + glutathionylspermidine + ATP $${\displaystyle \rightleftharpoons }$$ N1,N8-bis(glutathionyl)spermidine + ADP + phosphateThe 3 substrates of this enzyme are glutathione, glutathionylspermidine, … See more The active bifunctional enzyme of trypanothione synthase is found as a 74.4 KDa monomer consisting of 652 residues with two catalytic domains. Its C-terminal domain is a synthetase and has an ATP-grasp family fold … See more The main function of trypanothione synthase is to use the free energy generated from ATP hydrolysis to conjugate glutathione and spermidine to form the intermediate of glutathionylspermidine and then the final product of … See more The regulation of trypanothione synthase is currently thought to be driven by conformational changes caused by allosteric … See more The current believed mechanism for synthetase activity is that first glutathione and Mg -ATP bind to the enzyme in a ternary complex where glutathione becomes activated by ATP and forms glutathionyl phosphate. ADP then leaves the active site and … See more Many diseases such as Human African trypanosomiasis, Nagana disease in cattle, and Chagas disease are caused by kinetoplastid … See more WebK01833 TRYS; trypanothione synthetase/amidase 6. Ligases 6.3 Forming carbon-nitrogen bonds 6.3.1 Acid-D-ammonia (or amine) ligases (amide synthases) 6.3.1.9 trypanothione …
WebJun 26, 2024 · Figure 1. Trypanothione synthetase inhibitors. The structure and biological activities (IC 50 against TryS or K i, EC 50 against trypanosomatids and selectivity index) …
WebTrypanothione synthetase (TryS) catalyzes the biosynthesis of glutathione-spermidine adduct trypanothione (T(SH) 2) that is the principal intracellular thiol-redox metabolite in … song lyrics to do i hear a waltzWebMar 21, 2024 · Abstract. Trypanothione synthetase (TryS) catalyses the synthesis of N 1,N 8-bis(glutathionyl)spermidine (trypanothione), which is the main low molecular mass thiol … smallest lathe for gunsmithingWebJan 1, 2024 · Trypanothione synthetase (TryS) is a trypanosomatid-specific enzyme that catalyzes the two-step synthesis of trypanothione (T(SH) 2; N 1,N 8-bis(glutathionyl)spermidine), from two molecules of glutathione (GSH; L-γ-Glu-Cys-Gly) and a spermidine core along with ATP consumption . song lyrics to footlooseWebFeb 25, 2013 · The trypanothione synthetase (TryS) catalyses the two-step biosynthesis of trypanothione from spermidine and glutathione and is an attractive new drug target for … song lyrics to for the first timeWebJul 15, 2024 · Trypanosomatid-caused diseases are among the neglected infectious diseases with the highest disease burden, affecting about 27 million people worldwide … smallest laptop with optical driveWebOct 10, 2005 · Trypanothione plays a pivotal role in defence against chemical and oxidant stress, thiol redox homoeostasis, ribonucleotide metabolism and drug resistance in … smallest laser all in oneWebDec 8, 2011 · Trypanothione synthetase (TryS) is essential for the survival of the protozoan parasite Trypanosoma brucei, which causes human African trypanosomiasis.It is one of … song lyrics to gone too soon by daughtry