Phosphinothricin acetyltransferase gene

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August undefined, 2024

WebDownload scientific diagram Schematic structure of the T-DNA region of the binary vector pCAMBIA3301. LB, left border; RB, right border; bar, phosphinothricin acetyltransferase gene; gus-int, β ... Webmechanism of Agrobacterium-mediated gene transfer has been widely utilized to genetically modify mono-cots and dicots (Hansen and Wright, 1999; Fukuoka et al., 2000). Agrobacterium tumefaciensis a soil-borne pathogen that has the capacity to infect plants and transfer a segment of DNA (T-DNA) from its tumor-inducing (Ti) plasmid to the plant …

2.3.1.183: phosphinothricin acetyltransferase - BRENDA

WebJan 1, 1995 · Phosphinothricin is an inhibitor of glutamine synthetase, which plays a central role in the assimilation of ammonia and in the regulation of the nitrogen metabolism in plants. 9, 10 It is the only enzyme that detoxifies ammonia produced during nitrate reduction, photorespiration, and amino acid degradation in plant cells. WebNov 27, 2024 · Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also … the pretender not even a mouse

Brachypodium T-DNA Collection - DOE Joint Genome Institute

Webexpression cassette encodes a phosphinothricin acetyltransferase gene from Streptomyces . viridochromogenes . that is codon-optimized for expression in corn. Method for conferring WebInactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector … WebTranslations in context of "phosphinothricine acétyltransférase" in French-English from Reverso Context: Un gène bactérien codant pour la phosphinothricine acétyltransférase, une enzyme qui inactive le glufosinate-ammonium par acétylation, a été introduit dans la lignée HCR-1 par un croisement interspécifique avec la lignée transformée T45 de B. napus. the pretender potato head blues

pat: Genes List GM Approval Database - ISAAA.org

Schematic structure of the T-DNA region of the binary vector ...

WebGene: pat. Gene Source: Streptomyces viridochromogenes. Product: phosphinothricin N-acetyltransferase (PAT) enzyme. Function: eliminates herbicidal activity of glufosinate … WebStrauch, E.; Wohlleben, W.; Puhler, A.: Cloning of a phosphinothricin Nacetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli. Gene, 63, 65–74 (1988) CrossRef CAS PubMed Google Scholar the pretender movies dvdWebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. sight and sound jesus movie

"WebApr 23, 2012 · The phosphinothricin expression cassette consists of the NOS promoter (promoter of the A. tumefaciens nopaline synthase gene), the pat cds (phosphinothricin acetyltransferase gene from Streptomyces viridochromogenes), and the NOS terminator (terminator of the A. tumefaciens nopaline synthase gene). " - Phosphinothricin acetyltransferase gene

Phosphinothricin acetyltransferase gene

GmGSTU23 Encoding a Tau Class Glutathione S-Transferase …

WebDroege, W.; Broer, I.; Puehler, A.: Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide l-phosphinothricin (glufosinate) … WebAug 24, 2024 · The appearance of weeds, resistant to EPSP synthase (EPSPS) inhibitors, acetolactate synthase (ALS), synthetic auxins, and photosystem II (PSII) inhibitors, have caused farming difficulties. Therefore, we have taken a genetic approach to herbicide resistance as a developing method in these GM crops.

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WebIt codes for phosphinothricin acetyl transferase (PAT) proteins of 183 amino acids and shows 85% DNA sequence homology with another marker gene pat isolated from Streptomyces viridochromogenes. WebCloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli Gene 63 65-74 1988 Streptomyces viridochromogenes, Streptomyces viridochromogenes Tue494 3290054. brenda. 673843.

WebThe gene encodes phosphinothricin acetyltransferase, which N-acetylates phosphinothricin using acetyl-CoA. Figure 6.1 . Overview of biosynthetic pathways of a number of … WebFeb 1, 2014 · The genes encoding for bialaphos resistance (phosphinothricin acetyltransferase), originally cloned from bialaphos producing streptomycetes [ 3, 56, 59, 63 ], have been used as selection markers in plant biology and engineering [ 11 ].

WebMar 18, 2016 · Gene: BASTA selectable marker from Streptomyces hygrosopicus- phosphinothricin acetyltransferase gene to confer BASTA resistance as a selectable marker Promoter: 35S promoter from Cauliflower mosaic caulimovirus – CaMV 35S constitutive promoter to drive expression of GUS reporter gene Webphosphinothricin-N-acetyltransferase Test. SKU: GENE-PHOS-NAT Category: Foody Safety Assays Tag: plantExtract. Related products. urinalysis test …

WebPhosphinothricin N-acetyltransferase. Gene. bar. Status. UniProtKB reviewed (Swiss-Prot) Organism. Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) Amino acids. 171. Protein existence. Evidence at protein level. Annotation score. 3/5. sight and sound jobsWebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ... the pretender nbcWebJun 15, 1992 · Cloning of a phosphinothricin N- acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Streptomyces lividans and Escherichia coli Gene (1988) P.G. Reddy et al. Cloning and expression in Escherichia coli of an esterase-coding gene from the oil-degrading bacterium Acinobacter calcoaceticus RAG-1 Gene … sight and sound jonah archiveWebApr 1, 2024 · Only few glufosinate-tolerant genes, such as phosphinothricin acetyltransferase ( PAT) and bialaphos resistance ( bar) identified from Streptomyces, are currently available for developing genetically modified rice in agricultural application. sight and sound jesus dvdWebGene: pat Gene Source: Streptomyces viridochromogenes Product: phosphinothricin N-acetyltransferase (PAT) enzyme Function: eliminates herbicidal activity of glufosinate (phosphinothricin) herbicides by acetylation GM Trait: Glufosinate herbicide tolerance Events with gene pat the pretender netflixWebThe chimeric hygromycin phosphotransferase gene ( hph) and phosphinothricin acetyltransferase gene ( bar) have been used as selectable markers. The integration and expression of an hph gene in … the pretender piano sheet musicWebWohlleben W, Arnold W, Broer I, Hillemann D, Strauch E, Pühler A (1988) Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes TÜ494 and its expression in Nicotiana tabacum. Gene … the pretender mr lee